Improvement of HPLC in Concentration Determination of Lamotrigine in Human Serum
10.6039/j.issn.1001-0408.2015.32.14
- VernacularTitle:HPLC法测定人血清中拉莫三嗪浓度的方法改进
- Author:
Chunmei CHEN
;
Jianfeng LAI
;
Zhiqiang CHEN
- Publication Type:Journal Article
- Keywords:
Lamotrigine;
Therapeutic drug monitoring;
HPLC
- From:
China Pharmacy
2015;(32):4504-4505
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a rapid method for the concentration determination of lamotrigine (LTG) in human se-rum. METHODS:HPLC was adopted after serum samples treated with methanol. The column was Hypersil BDS C18(200 mm×4.6 mm,5μm)with the mobile phase of methanol-KH2PO4(45∶55,V/V)at the flow rate of 1 ml/min,the detection wavelength was 250 nm,and the temperature was 35℃. RESULTS:The linear range of LTG was 0.5-30.0 μg/ml(r=0.999 9). The RSDs of intra-day and inter-day were all less than 5% and the recovery was 101.96%-102.74%. CONCLUSIONS:The method is simple,accurate and fast,and suitable for the monitoring of LTG serum concentration.
