Content Determination of Sinomenine in Qihuang Capsule by HPLC
10.6039/j.issn.1001-0408.2015.30.23
- VernacularTitle:HPLC法测定芪黄胶囊中青藤碱的含量
- Author:
Yong YANG
;
Weizao LUO
;
Xuming LIANG
;
Weihan QIN
;
Dashuang QING
;
Dejiang WANG
;
Ying WANG
- Publication Type:Journal Article
- Keywords:
Qihuang capsule;
Sinomenine;
Content;
HPLC
- From:
China Pharmacy
2015;(30):4243-4244
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of sinomenine in Qihuang capsule. METHODS:HPLC was performed on the column of welch C18-AQ with mobile phase of methanol-phosphate buffer(gradient elution) at flow rate of 1.0 ml/min,detection wavelength was 264 nm,column temperature was 30 ℃ and volume injection was 10 μl. RESULTS:The linear range of sinomenine was 0.200 3-10.016 0 μg(r=0.999 8),RSDs of precision,stability and reproducibility tests were lower than 1.0%,recovery was 98.80%-100.94%(RSD=0.79%,n=6). CONCLUSIONS:The method is simple,accurate and re-producible,and can be used for the content determination of sinomenine in Qihuang capsule.
