Contents Determination of Harpagoside and Stilbene Glycoside in Shuangshen Xiaolong Granule by HPLC
10.6039/j.issn.1001-0408.2015.30.28
- VernacularTitle:HPLC法测定双参消癃颗粒中哈巴俄苷和二苯乙烯苷的含量
- Author:
Jing ZHOU
;
Weijie HUANG
;
Subei YANG
;
Rusong ZHANG
- Publication Type:Journal Article
- Keywords:
HPLC;
Shuangshen xiaolong granules;
Harpagoside;
Stilbene glycoside;
Content determination
- From:
China Pharmacy
2015;(30):4255-4257
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the contents determination of harpagoside and stilbene glycoside in Shuang-shen xiaolong granule. METHODS:HPLC of harpagoside was performed on the column of Kromasil 100-5 C18 with mobile phase of acetonitrile-1% acetic acid solution (gradient elution) at flow rate of 1.0 ml/min,detection wavelength was 278 nm,column temperature was 25 ℃ and volume injection was 20 μl. HPLC of stilbene glycoside was performed on the column of Kromasil 100-5 C18 with mobile phase of acetonitrile-water(19∶81,V/V)at flow rate of 1.0 ml/min,etection wavelength was 320 nm,column temperature was 25 ℃ and volume injection was 10 μl. RESULTS:The linear range was 0.555 8-8.893 4 μg for harpagoside(r=0.999 9)and 0.010 6-0.340 2 mg for stilbene glycoside(r=0.999 6);RSDs of precision,stability and reproducibility tests were no more than 1.80%;recoveries were 97.30%-101.35%(RSD=1.43%,n=6) and 96.67%-100.83%(RSD=1.48%,n=6),respec-tively. CONCLUSIONS:The method is simple,accurate and reproducible,and can be used for the contents determination of harpa-goside and stilbene glycoside in Shuangshen xiaolong granule.
