Quality Evaluation of Fructus aurantii and Citrus Changshan-huyou with Their Processed Products
10.6039/j.issn.1001-0408.2015.30.29
- VernacularTitle:枳壳与常山胡柚及其多个炮制品的质量评价Δ
- Author:
Jianfeng SONG
;
Jingqian FENG
;
Liping XU
;
Weinan HU
;
Chuang CHENG
;
Chunxian ZHU
;
Yichao FANG
;
Xinli CHAI
;
Siqing ZHAO
;
Jianhua HU
;
Jianli ZHENG
- Publication Type:Journal Article
- Keywords:
HPLC;
Fructus aurantii;
Processed products;
Naringin;
Neohesperidin;
Content determination
- From:
China Pharmacy
2015;(30):4258-4261
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To determine the mass scores of naringin and neohesperidin in Fructus aurantii and Citrus chang-shan-huyou with their processed products and evaluate the quality of Fructus aurantii and Citrus changshan-huyou with their pro-cessed products. METHODS:According to the requirements of Chinese Pharmacopoeia(2010 edition)and Zhejiang Province Tradi-tional Chinese Medicine Preparation Standards (2005 edition),the moisture and ash of F. aurantii and C. changshan-huyou with their processed products were detected. And the contents of naringin and neohesperidin were determined. The ZORBAX SB-C18 column was used with the mobile phase of acetonitrile-water(20∶80,V/V)at the flow rate of 1.0 ml/min. The detection wave-length was set at 283 nm,and the column temperature was 40℃.The samples size was 10μl. RESULTS:The moisture of F. au-rantii and C. changshan-huyou was decreased after processing with no obvious change for ash. The contents of naringin and neohes-peridin were decreased,significantly for F. aurantii,and all consistent with the requirements of Chinese Pharmacopoeia(2010 edi-tion)except F. aurantii. The linear range was 0.028 45-0.284 5μg(r=0.999 7)for naringin and 0.085 9-0.858 6μg(r=0.999 6)for neohesperidin;the RSDs of precision,stability and reproducibility tests were no more than 1.36% and the average recovery was re-spectively 96.45%-100.43%(RSD=1.45%,n=6) and 98.36%-102.00%(RSD=1.26%,n=6). CONCLUSIONS: There was no significant difference in the inspection and determination re-sults in F. aurantii and C. changshan-huyou. It is suggested to adjust the limitation of content determination in the Chinese Pharmacopoeia(2010 edition)and processed standards.
