Simultaneous Determination of Glycyrrhizic Acid and Glycyrrhetinic Acid in Yaotongning Capsule by HPLC
10.6039/j.issn.1001-0408.2015.30.37
- VernacularTitle:HPLC法同时测定腰痛宁胶囊中甘草酸和甘草次酸的含量
- Author:
Yanning HE
;
Yinli ZHAO
;
Dongli YANG
;
Dongge ZHANG
;
Chunmin WANG
- Publication Type:Journal Article
- Keywords:
Yaotongning capsule;
Glycyrrhizic acid;
Glycyrrhetinic acid;
HPLC
- From:
China Pharmacy
2015;(30):4279-4281
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for simultaneously determination of glycyrrhizic acid and glycyrrhetinic acid in Yaotongning capsule. METHODS:HPLC was performed on the column of Agilent TC-C18 with mobile phase of methanol-0.2 mol/L ammonium acetate(gradient elution) at a flow rate of 1.0 ml/min;detection wavelength was 250 nm and column temperature was 25 ℃ and injection volume was 10 μl. RESULTS:The linear range was 0.007 1-0.178 0 mg/ml(r=0.999 8)for glycyrrhizin acid and 0.354 8-8.720 0 μg/ml of glycyrrhetinic acid(r=0.999 8);RSDs of precision,stability and reproducibility tests were no more than 1.74%,average recoveries were 95.49%-100.62%(RSD=1.98%,n=9)and 96.80%-102.26%(RSD=1.83%,n=9),respec-tively. CONCLUSIONS:The method is simple,reproducible,accurate and reliable,and can be used for the simultaneous determi-nation of glycyrrhizic acid and glycyrrhetinic acid in Yaotongning capsule.
