Effects of Indometacin on Apoptosis and Proliferation of Cervical Cancer Hela Cell
10.6039/j.issn.1001-0408.2015.31.18
- VernacularTitle:吲哚美辛对宫颈癌Hela细胞增殖和凋亡作用的影响
- Author:
Hui XU
;
Jia YU
;
Liangzhong LYU
- Publication Type:Journal Article
- Keywords:
Indometacin;
Cervical cancer Hela cell;
Proliferation;
Apoptosis
- From:
China Pharmacy
2015;26(31):4375-4377
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effects of indometacin on apootosis and proliferation of cervical cancer Hela cell. METH-ODS:Hela cell was cultured in vitro as study object,and cultured with 0(blank control),200,400,600,800 and 1 000 μmol/L indometacin for 24,48 and 72 h. The inhibitory rate of indometacin to the proliferation of Hela cells was detected by MTT assay. After treated with 0(blank control),400,600 and 800 μmol/L indometacin for 24 h,the change of cellular morphology was ob-served by invert microscope;cell cycle phase and apoptosis were analyzed by flow cytometry. RESUITS:Indometacin of 600, 800,1 000μmol/L could inhibit the proliferation of Hela cell,which was positively correlated to drug concentration and time. Com-pared with blank control,indometacin could induce that Hela cell transformed from polygonous to round in appearance,and result-ed in cell apoptosis and necrosis;the proportion of cells at G0/G1 phase increased,while the proportion of cells at S phase reduced;the apoptotic rate of cells raised. CONCLUSIONS:Indometacin could inhibit the proliferation of Hela cell,block cell cycle at G0/G1 phase and induce apoptosis.
