Study on Purification Technology of Total Alkaloid from the Flos of Aconitum kusnezoffii
10.6039/j.issn.1001-0408.2015.31.25
- VernacularTitle:草乌花总生物碱的纯化工艺研究
- Author:
Xiaotong LIN
;
Na GUO
;
Ling ZHOU
;
Weijian HAN
;
Jia CAO
;
Tingting ZHANG
;
Nan XU
- Publication Type:Journal Article
- Keywords:
Flos of Aconitum kusnezoffii;
Total alkaloids;
Content determination;
Cation exchange resin;
Purification technology
- From:
China Pharmacy
2015;26(31):4396-4398
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimize the purification technology of total alkaloid from the flos of Aconitum kusnezoffii. METH-ODS:The content of total alkaloid from the flos of A. kusnezoffii was determined by acid-base titration. The purification technology of total alkaloid from the flos of A. kusnezoffii was optimized by ion resin with resin type,mass concentration of loading liquid and exchange speed as factors,maximum adsorption quantity,desorption rate and mass fraction of total alkaloid as index,and verifica-tion test was conducted. RESULTS:The optimal purification technology was as follows as type 732 cation exchange resin,mass concentration of loading liquid 0.32 g/L,exchange speed of 7 column volume(BV)/h. In validation test,the content of total alka-loid was 86.88%(RSD=0.52%,n=3),and desorption rate was 92.81%(RSD=0.40%,n=3)averagely. The extraction trans-port rate of total alkaloid from 3 batches of the flos of A. kusnezoffii was 81.76% and purification transport rate was 89.47% in av-erage. CONCLUSIONS:The established method is stable and feasible,and shows high transport rate.
