Comparative Study of Genetic Diversity of Spatolobi caulis from Guangxi by RAPD and ISSR Method
10.6039/j.issn.1001-0408.2015.31.10
- VernacularTitle:广西产鸡血藤遗传多样性的RAPD与ISSR标记方法的比较研究
- Author:
Hui TIAN
;
Changyue JIANG
;
Hua ZHU
;
Xiaoxun WANG
;
Min ZHOU
;
Jian CUI
;
Yi FENG
- Publication Type:Journal Article
- Keywords:
Spatolobi caulis from Guangxi;
Random amplified polymorphic DNA;
Inter-simple sequence repeat;
Genetic diversity
- From:
China Pharmacy
2015;26(31):4348-4350
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To compare genetic diversity of Spatolobi caulis from different areas of Guangxi by random amplified polymorphic DNA(RAPD)and inter-simple sequence repeat(ISSR). METHODS:Through using POPGENE 32 software,Ntsys software and SPSS 17.0 software,RAPD and ISSR methods were used to study genetic diversity of 9 samples of S. caulis from dif-ferent areas of Guangxi. RESULTS:After amplification of screened 3 RAPD primers and 4 ISSR primers,and there were 198 and 315 locus,and 37 and 80 polymorphism locus. Rates of polymorphism locus were 18.7% and 25.4%;the number of effective al-leles were 1.416 8 and 1.584 0;genetic diversity index were 0.269 4 and 0.351 3;Shannon diversity index were 0.431 6 and 0.529 9. All the values of ISSR marker were higher than RAPD marker. The average genetic similarity coefficient of ISSR and RAPD were 0.757 64 and 0.683 80,indicating ISSR was more sensitive for the detection of genetic diversity. The clustering result of them was close to each other. The correlation coefficient of them were 0.847,indicating very significant positive correlation at the level of 0.001. CONCLUSIONS:ISSR could reflect more information of genetic diversity than RAPD,and is more suitable for research of genetic diversity of S. caulis from different areas of Guangxi.
