Screening and Identification of Endophytic Fungi from Schisandra chinensis with Antioxidant Activity
10.6039/j.issn.1001-0408.2015.31.21
- VernacularTitle:具有抗氧化活性的北五味子内生真菌的筛选及鉴定
- Author:
Yue ZHAO
;
Yuan QIN
;
Na LI
;
Zhipeng LI
;
Zhiqiang FAN
;
Xiangyong YU
;
Jingming JIA
- Publication Type:Journal Article
- Keywords:
Schisandra chinensis;
Endophytic fungi;
Antioxidant activity;
Identification;
Clonostachys rosea
- From:
China Pharmacy
2015;26(31):4384-4388
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To screen and identify endophytic fungi from Schisandra chinensis with antioxidant activity. METH-ODS:The tissue isolation skill was used to isolate endophytic fungi from roots,leaves,stems and fruits of S. chinensis. And anti-oxidant activity of endophytic fungi were screened by DPPH radical scavenging assay and hydroxyl radical scavenging assay. The to-tal DNA were extracted;the 18S rDNA ITS were amplified and sequenced with primer ITS1 and ITS4;the results of sequencing were analyzed comparatively based on homology to confirm the classification of active strains. RESULTS:23 strains were isolated from S. chinensis. GSR-12,isolated from roots of S. chinensis,had strong antioxidant activities. The scavenging rate on DPPH and the hydroxyl radical were 87.96% and 82.31% respectively. GSR-12 strain was identified as Clonostachys rosea by analyzed com-paratively. CONCLUSIONS:1 strain of C. rosea,isolated from roots of S. chinensis,has strong antioxidant activity.
