Study on Purification Technology of Total Flavonoids from Nelumbinis receptaculum by Macroporous Resin
10.6039/j.issn.1001-0408.2015.31.28
- VernacularTitle:大孔树脂纯化莲房总黄酮的工艺研究
- Author:
Shuxia ZHENG
;
Jun YI
;
Jinzhong WU
;
Chunjiang TAN
;
Jianguo WU
;
Jianzhong CHEN
;
Yanbin WU
- Publication Type:Journal Article
- Keywords:
Nelumbinis receptaculum;
Total flavonoids;
Macroporous resin;
Purification technology
- From:
China Pharmacy
2015;26(31):4405-4408
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the purification technology of total flavonoids from Nelumbinis receptaculum by macropo-rous resin. METHODS:Using adsorption rate and desorption rate of total flavonoids from Nelumbinis receptaculum as index,the type of macroporous resin was selected by static adsorption-desorption tests;With adsorption rate of total flavonoids as index,sin-gle factor test was used to investigate the effects of the concentration of total flavonoids,adsorption time,adsorption speed, drug-loading amount,water amount,volume fraction and amount of eluant and other factors on the purification technology. The op-timal technology was validated. RESULTS:Among 10 kinds of resin,HPD-400 macroporous resin was found to have the best ad-sorption and desorption effects. The optimal purification conditions was as follows as the concentration of total flavonoids 7.00 mg/ml, adsorption time of 3 h,flow rate for sampling of 3 column volume (BV)/h,drug-loading amount of 8 BV,water amount of 6 BV,50% ethanol elution amount of 4 BV. In validation test,mass fraction of total flavonoids from purified Nelumbinis receptacu-lum were 63.88%,62.50% and 63.44%(RSD=1.11%,n=3). CONCLUSIONS:HPD-400 macroporous resin could purify total flavonoids from purified Nelumbinis receptaculum,and established purification technology is stable and practical.
