Optimization of the Extraction Technology of Total Saponins from Coreopsis tinctoria by Orthogonal Test
10.6039/j.issn.1001-0408.2015.31.31
- VernacularTitle:正交试验优选昆仑雪菊总皂苷的提取工艺
- Author:
Houhe LIU
;
Zhen WANG
;
Zhaoming LIU
;
Yabo SHI
;
Junpeng GAO
- Publication Type:Journal Article
- Keywords:
Coreopsis tinctoria;
Total saponins;
Single factor experiment;
Orthogonal test;
Extraction technology
- From:
China Pharmacy
2015;26(31):4415-4417
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimize the extraction technology of total saponins from Coreopsis tinctoria. METHODS:Etha-nol leaching technology was adopted. Based on single factor test,the extraction technology was optimized by orthogonal test using extraction temperature,ethanol volume fraction,extraction time,solid-liquid ratio as factors,extraction rate of total saponins as in-dex. The optimized technology was validated. RESULTS:The optimal technology was that ratio of solid to liquid was 1:30 (C. tinctoria-60% ethanol),extracting for 2 h at 50 ℃. Validation test showed that average extraction rate of total saponins was 6.8%(RSD=0.85%,n=3). CONCLUSIONS:The optimized technology can be used for the extraction of total saponins from C. tinc-toria and keep stable.
