Optimization of Ultrasonic-assisted Extraction Technology of Total Flavonoids from Vaccinium uliginosum
10.6039/j.issn.1001-0408.2015.31.35
- VernacularTitle:超声波辅助提取蓝莓总黄酮的工艺优化
- Author:
Jianhua CAO
;
Guanghui LI
;
Min ZHANG
- Publication Type:Journal Article
- Keywords:
Vaccinium uliginosum;
Total flavonoids;
Yield;
Extraction technology;
Optimization;
Ultrasonic-assisted extrac-tion;
Orthogonal test
- From:
China Pharmacy
2015;26(31):4426-4428
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimize ultrasonic-assisted extraction technology of total flavonoids from Vaccinium uliginosum. METHODS:Based on single factor test,the ultrasonic-assisted extraction technology of total flavonoids from V. uliginosum was op-timized by orthogonal test,using the yield of total flavonoids as index,with ethanol volume fraction,solid-liquid ratio and ultrason-ic power as factors. The optimized technology was validated and compared with traditional ethanol reflux method(reflux extracting for 2 times,2 h each time). RESULTS:The optimal extraction technology was as follows as the solid-liquid ration of 1:25,70%ethanol,ultrasonic power of 250 W(40 min for ultrasonic time). In validation test,the yield of total flavonoids from V. uliginosum was 3.98%(RSD=0.41%,n=3),and the yield of total flavonoids was 2.25% by ethanol reflux method. CONCLUSIONS:The ultrasonic-assisted extraction is obviously superior to ethanol reflux method on extraction time and effects,and it is suitable for in-dustrialized production.
