Screening of Antioxidant Active Components of Schisandra chinensis
10.6039/j.issn.1001-0408.2016.19.10
- VernacularTitle:五味子抗氧化活性组分筛选
- Author:
Yinping JIN
;
Wei HOU
;
Wei GAO
;
Junxia LIU
;
Yushuai WANG
;
Shi YAN
;
Yingping WANG
- Publication Type:Journal Article
- Keywords:
Schisandra chinensis;
Antioxidation;
Active components;
Extraction technology;
Orthogonal test;
Eluate;
DPPH free radical scavenging activity;
Total antioxidant ability
- From:
China Pharmacy
2016;27(19):2622-2625
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To screen antioxidant active components of Schisandra chinensis. METHODS:The orthogonal test was adopted to optimize extraction technology using DPPH free radical scavenging activity(IC50)as index and ethanol volume frac-tion,material-liquid ratio and extraction time as factors,and the verification test were made. The fractions(SC-0,SC-10,SC-30, SC-50,SC-70,SC-95) were made by extracting and purifying S. chinensis with macroporous resin with water and 10%,30%, 50%,70%and 95%ethanol. With IC50 and total antioxidant capacity(determined by ABTS method)as indexes(vitamin C as pos-itive control),the antioxidant active components of S. chinensis were optimized. The contents of 5 kinds of lignan in different posi-tions of S. chinensis were determined by HPLC. RESULTS:The optimal extraction condition of S. chinensis was as follows as 60% ethanol,material-liquid ratio of 1∶14,extracting for 2.0 h. The average IC50 of DPPH free radical scavenging activity was 23.81 mg/ml(RSD=0.52%,n=3)in verification test. SC-0 did not have antioxidant abilities. DPPH free radical scavenging activi-ty of those components (ie. the IC50 value from low to high) were in the following order of positive control>SC-50>SC-30>SC-95>SC-70>SC-10;total antioxidant ability of them were in the following order of SC-50>positive control>SC-30>SC-70>SC-95>SC-10;the contents of 5 types of lignan in different components were in the following order of SC-70>SC-50>SC-95>SC-30. CONCLUSIONS:The antioxidant active component of S. chinensis is 50%ethanol eluate.
