Study on Dermal Pharmacokinetic Difference of Triptolide in Normal and Diabetic Rats by Microdialysis
10.6039/j.issn.1001-0408.2016.19.16
- VernacularTitle:采用微透析法研究雷公藤甲素在正常和糖尿病大鼠皮肤中的药动学差异
- Author:
Ziping ZHANG
;
Weidong ZENG
;
Xiucai GUO
;
Boxin ZHAO
;
Yingguang DENG
- Publication Type:Journal Article
- Keywords:
Triptolide;
Microdialysis;
Dermal pharmacokinetics;
Diabetes;
Percutaneous penetration amount;
Rats
- From:
China Pharmacy
2016;27(19):2641-2643
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the dermal pharmacokinetic difference of triptolide in normal and diabetic rats,and to pro-vide reference for rational drug use in the clinic. METHODS:12 Wistar rats were randomly divided into normal group and diabetic model group(0.1%streptozotocin intraperitoneally),with 6 rats in each group. Both group were given Triptolide cream 0.5 g to ab-dominal skin,and dialysate was collected by microdialysis every 30 min for consecutive 12 h. Subcutaneous concentration was de-tected by HPLC-MS,and subcutaneous concentration-time curves were analyzed and compared between two groups,and Winnon-lin 5.0.1 software was used to calculate pharmacokinetic parameters. RESULTS:The pharmacokinetic parameters of normal group and diabetic model group were that cmax were(1.54±0.37)and(5.12±1.34)μg/ml;tmax were(7.32±0.24)and(6.21±0.35)h;AUC0-12 h were (12.65 ± 4.64) and (37.43 ± 5.23)μg·h/ml,with statistical significance (P<0.05). CONCLUSIONS:The change of dermal structure caused by diabetes can increase percutaneous penetration amount of triptolide in rats,and drug dosage should be reduced according to circumstances so as to reduce side effects.
