Study on Optimization of Ultrasonic-assisted Extraction Technology for Total Flavonoids from Tibetan Scindapsus aureus by Response Surface Method
10.6039/j.issn.1001-0408.2015.25.36
- VernacularTitle:响应面法优化藏药绿萝花总黄酮的超声波辅助提取工艺
- Author:
Xiaojun YANG
;
Yonghui DING
- Publication Type:Journal Article
- Keywords:
Tibetan Scindapsus aureus;
Total flavonoids;
Ultrasonic-assisted extraction;
Box-Behnken central component de-sign;
Response surface method;
Technology optimization
- From:
China Pharmacy
2015;(25):3565-3568
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimize the ultrasonic-assisted extraction technology of total flavonoids from Tibetan Scindapsus aureu. METHODS:The single-factor test was adopted to investigate the effects of extraction time,extraction temperature,the vol-ume fraction of ethanol,the ratio of liquid to solid and power on the extraction rate of total flavonoids from Tibetan Scindapsus au-reu in the process of ultrasonic-assisted extraction,based on which,four-factor and five-level Box-Behnken central component de-sign was performed with unchanged ultrasonic power,and then response surface method was adopted to analyze the effects of the 4 factors and their interaction on the extraction rate of total flavonoids to determine the optimal factor and level,and verification tests were conducted.RESULTS:The optimal ultrasonic-assisted extraction technology was as follows as extraction time of 29 min,tem-perature of 44 ℃,volume fraction of ethanol of 78%,ratio of liquid to solid of 13∶1(ml/g). In three verification tests,the aver-age extraction rate of total flavonoids was 2.130%,with an relative error of 0.005% compared to the theoretical value. CONCLU-SIONS:The optimal technology is reasonable and feasible,and can be used for theultrasonic-assisted extraction of total flavonoids from Tibetan Scindapsus aureu.
