Optimization of Decoction Process of Agkistrodon by Orthogonal Experiment
10.6039/j.issn.1001-0408.2015.25.37
- VernacularTitle:正交试验优选蕲蛇煎煮工艺
- Author:
Shiwei CHAI
;
Gaiying DONG
;
Jingtian QU
;
Ran WANG
- Publication Type:Journal Article
- Keywords:
Agkistrodon;
Amino acids;
RP-HPLC;
Pre-column derivatization;
Water decoction;
Technology optimization;
Or-thogonal experiment
- From:
China Pharmacy
2015;(25):3569-3571
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimize the decoction process of agkistrodon. METHODS:RP-HPLC with pre-column derivatiza-tion was adopted. With the contents of 4 main amino acids in agkistrodon as index,the decoction process(decocting times,heating time,water quantity and medicinal material crushing granularity) was optimized by orthogonal tests and verified. RESULTS:The optimal technology of the decoction process of agkistrodon was as 3 times of decoction,60 min of heating time,50 ml of water consumption for 0.90 g medicinal material and No.6 sieve. The results of verification test showed the total extraction of 4 kinds of amino acids was 72.68 mg/g(RSD=3.77%,n=3). CONCLUSIONS:The decoction technology can be used for the decoction pro-cess of agkistrodon,and it is stable and feasible.
