The Effects of Scorpion’s Proteins with Different Molecular Weights on Angiogenesis of the Transgenic Ze-brafish
10.6039/j.issn.1001-0408.2015.25.12
- VernacularTitle:不同分子质量段全蝎蛋白对转基因斑马鱼血管生成的影响
- Author:
Lin HOU
;
Qinqin ZHOU
;
Qinghua CUI
;
Jingzhen TIAN
- Publication Type:Journal Article
- Keywords:
Scorpion;
Ultrafiltration;
Chromatography;
Zebrafish;
Angiogenesis
- From:
China Pharmacy
2015;(25):3490-3492
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effects of scorpion’s proteins with different molecular weights on angiogenesis of the transgenic zebrafish. METHODS:The vascular fluorescence transgenic zebrafish models were established. Scorpion’s proteins were separated by ultrafiltration and ion exchange chromatography to obtain the scorpion protein fractions with different molecular weights (3-10 ku,>10-50 ku and>50 ku). The embryos of transgenic zebrafishes were cultured in the above 10,100 and 500 μg/ml scor-pion’s proteins. Intersegmental vessels of the transgenic zebrafishes were counted under the fluorescence microscope to optimize the most suitable scorpion’s protein molecular weight. The vessels were counted again with >50 ku scorpion protein component 1 and 2,so as to select suitable component.RESULTS:The >50 ku scorpion’s protein fraction component 1 with the mass concentration of 500 μg/ml had the highest inhibitory activity for the angiogenesis of the transgenic zebrafish,with inhibitory rate of 92.59%. CONCLUSIONS:Scorpion’s protein and its fractions have the activity of angiogenesis inhibition,which may be one of anti-cancer mechanisms of scorpion.
