Optimization of the Ultrasonic Extraction Process of Total Alkaloids from Mulberry Leaves by Response Surface Method
10.6039/j.issn.1001-0408.2015.25.28
- VernacularTitle:响应面法优化桑叶总生物碱超声提取工艺
- Author:
Sheng HE
;
Xingzi ZHOU
;
Hai HE
;
Xianxia HE
;
Huajing CHANG
;
Xinrong WU
- Publication Type:Journal Article
- Keywords:
Mulberry leaf;
Total alkaloids;
Response surface method;
Ultrasonic extraction;
Optimization
- From:
China Pharmacy
2015;(25):3537-3540
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimize the ultrasonic extraction process of total alkaloids from mulberry leaves. METHODS:Based on the single factor experiment,response surface method was adopted to investigate the effects of ultrasonic extraction time, ultrasonic power,ethanol volume fraction,solid-liquid ratio and pH of solvent on the extraction rate of total alkaloids from mulber-ry leaves,then test data were analyzed by Design Expert 8.0.5 software,and the optimized process was confirmed and verified. RE-SULTS:The multiple correlation coefficient of established binomial equation fitting model was 0.969 9;the optimized condition for extracting alkaloid from mulberry leaf was ultrasonic extraction time of 48 min,ultrasonic extraction power of 800 W,ethanol vol-ume fraction of 70%,material-liquid ratio of 1∶25,pH 5. Under these conditions,the extraction rate of total alkaloids was 0.422%, with the bias ratio was less than 2% compared with the model predictions. CONCLUSIONS:The established model has good fit-ting performance,the extraction process is stable and reliable,and can be used for the extraction of alkaloids from mulberry leaves.
