Preparation and Characterization of Celecoxib-loaded PLGA Nanoparticles
10.6039/j.issn.1001-0408.2015.25.35
- VernacularTitle:载塞来昔布-聚乳酸/羟基乙酸共聚物纳米粒的制备及表征
- Author:
Min WANG
;
Peng XIE
;
Yimin YANG
;
Qiuyan LI
;
Qiang XUE
- Publication Type:Journal Article
- Keywords:
Celecoxib;
PLGA;
Nanoparticles;
Emulsification-solvent evaporation method;
Plackett-Burman design;
Box-Behnk-en design
- From:
China Pharmacy
2015;(25):3561-3564
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To prepare and characterize celecoxib-loaded PLGA nanoparticles. METHODS:Emulsification-solvent evaporation method was adopted to prepare celecoxib-loaded PLGA nanoparticles. With encapsulation efficiency and particle size as the indexes,Plackett-Burman design was preferred to screen the formulation and variables which had a significant effect on the property of nanoparticles. And then Box-Behnken response surface method was used to further optimize selected variables including mass concentration of PLGA,ultrasonic power and ultrasonic time,followed by verification. Malvern particle size analyzer was used to determine the particle size distribution of nanoparticles and Zeta potential of nanoparticle by the optimal formulation technol-ogy,and transmission electron microscope was used to observe the morphology of the nanoparticles,and their drug release in vitro behavior and stability(25,5 ℃)were also observed. RESULTS:The optimal formulation and technology was as follows as PLGA mass concentration of 30.0%,ultrasonic power of 180 W and ultrasonic time of 8 min. For the prepared nanoparticles,encapsula-tion efficiency and particle size were (85.7 ± 4.1)% and (226.1 ± 36.1) nm (n=3) respectively;particle size distribution was (176.2±41.2)nm,polydispersity index was 0.211±0.021,and Zeta potential was(-37.3±1.6)mV. Under the electron micro-scope,the nanoparticles were homogeneous in particle size and distributed spheroidally,with 24 h accumulative release of 52.4%. They were stable within 3 months at 5℃. CONCLUSIONS:Celecoxib-loaded PLGA nanoparticles have been prepared successfully.
