Comparative Study on HPLC Characteristic Spectrum of Erycibe obtusifolia from Different Habitats by HPLC
10.6039/j.issn.1001-0408.2015.24.36
- VernacularTitle:不同产地丁公藤药材的HPLC特征图谱对比研究
- Author:
Jing WEN
;
Hongzhang SUN
;
Jinghua LU
- Publication Type:Journal Article
- Keywords:
Erycibe obtusifolia;
Characteristic spectrum;
HPLC
- From:
China Pharmacy
2015;(24):3417-3419
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish and compare the HPLC characteristic Spectrum Erycibe obtusifolia from different habi-tats. METHODS:HPLC was performed on the column of Hypersil ODS C18 with the mobile phase of acetonitrile-0.1% phosphoric acid at flow rate of 1.0 ml/min,detection wavelength was 347 nm,the column temperature was 30℃and the sample size was 20μl. 12 different batches of samples were determined and the characteristic spectrums of those were established. TCM fingerprint evalu-ation software(2004 edition)was used to evaluate the similarity of 12 batches of samples. RESULTS:10 characteristic peaks were identified in the characteristic spectrum of E. obtusifolia. Peak 3 was scopolamine glycosides,peak 4 was chlorogenic acid and peak 6 was scopoletin. The similarities of 10 batches of samples were proved to be higher than 0.90 and 2 batches were proved to be less than 0.90. CONCLUSIONS:The method is simple,accurate and reproducible,and can provide scientific evidence for effectively controlling the internal quality standards.
