Study on the Quality Standard of Huangmai Granules
10.6039/j.issn.1001-0408.2015.24.34
- VernacularTitle:黄麦颗粒的质量标准研究
- Author:
Guanqin JIN
;
Linghong XIA
;
Li SUN
;
Houwen LIN
- Publication Type:Journal Article
- Keywords:
Huangmai granules;
TLC;
HPLC;
Quality standard
- From:
China Pharmacy
2015;(24):3410-3413
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the quality control of Huangmai granules and provide reference for the qual-ity control. METHODS:TLC was conducted to identify the Leguminosae in Huangmai granules and HPLC was used to determine the contents of icariin,hyperin and stilbene glycoside in Huangmai granules. RESULTS:The spots on TLC plates were clear with good reproducibility. The results of HPLC showed that the linear range was 9.969-319.0 μg/ml(r=0.999 9) for icariin , 12.3-196.8 μg/ml(r=0.999 9)for hyperin and 12.64-202.2 μg/ml(r=0.999 8)for stilbene glycoside;RSDs of precision,stability and reproducibility tests were all no more than 2.92%;the average recoveries were respectively 95.44%(RSD=1.46%,n=6), 101.06%(RSD=1.90%,n=6)and 100.51%(RSD=1.73%,n=6). CONCLUSIONS:The method is simple,reproducible,accu-rate and reliable,and can be used for the quality control of Huangmai granules.
