Simultaneous Determination of the Content of Catalpol,Harpagide and Hapagoside in AIDS Oral Gargle by Double-wavelength UPLC
10.6039/j.issn.1001-0408.2015.24.35
- VernacularTitle:双波长UPLC法同时测定艾滋病口腔含漱液中梓醇、哈巴苷和哈巴俄苷的含量Δ
- Author:
Hui YANG
- Publication Type:Journal Article
- Keywords:
UPLC;
AIDS oral gargle;
Catalpol;
Harpagide;
Hapagoside;
Content determination
- From:
China Pharmacy
2015;(24):3414-3416
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for simultaneous determination of the content of catalpol,harpagide and hapa-goside in AIDS oral gargle. METHODS:Dual-wavelength UPLC was performed on the column of Eclipse Plus C18 with mobile phase of acetonitrile-0.05% phosphoric acid (gradient elution) at the flow rate of 0.2 ml/min. Wavelength was switching,it was set at 210 nm to determine the contents of catalpol and harpagide in the first 8 min and then changed to 280 nm to determine the con-tent of hapagosi- de in 8-15 min. The column temperature was 30 ℃,and injection volume was 2 μl. RESULTS:The linear range was 0.018 5-0.129 2 μg (r=0.999 6) for catalpol,0.014 0-0.098 3 μg (r=0.999 7) for harpagide and 0.015 7-0.110 0 μg(r=0.999 7)for hapagoside;RSDs of precision,stability and reproducibility tests were no more than 0.77%;average recoveries were respectively 99.44%(RSD=0.75%,n=6),99.90%(RSD=0.82%,n=6) and 100.20%(RSD=0.73%,n=6). CONCLUSIONS:The method is simple can reproducible,and can be used for effectively quality control.
