Determination of Related Substances in Methylacrylic Acid-Ethyl Acrylate Copolymer by HPLC
10.6039/j.issn.1001-0408.2015.24.43
- VernacularTitle:HPLC法测定甲基丙烯酸-丙烯酸乙酯共聚物中有关物质的含量
- Author:
Dong WANG
;
Jianpeng ZHOU
;
Chen WANG
- Publication Type:Journal Article
- Keywords:
Methyl acrylic acid-ethyl acrylate copolymer;
Related substances;
HPLC;
Content
- From:
China Pharmacy
2015;(24):3436-3438
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the determination of related substances in methylacrylic acid- ethyl acrylate copolymer. METHODS:HPLC was performed on the column of Agilent C18 with mobile phase of methanol-phosphate buffer(70∶30,V/V) at the flow rate of 1.0 ml/min,the detection wavelength was 202 nm,column temperature was 20 ℃,and the sample size was 20 μl. RESULTS:The linear range was 0.001%-0.015% for both methylacrylic acid(r=0.999 4)and ethyl acrylate(r=0.999 1);RSDs of precision,stability and reproducibility tests were lower than 2%;the average recoveries of methylacrylic acid and ethyl acrylate were respectively 86.59%(RSD=2.3%,n=9)and 91.24%(RSD=3.5%,n=9). CONCLUSIONS:The method is simple,accurate and reproducible,and can be used for the contents determination of related substances in methyl acrylic acid-eth-yl acrylate copolymer.
