Study of Liver Damage Mechanism in Mice Caused by the Incompatibility of Daphne genkwa Combined with Glycyrrhiza uralensis
10.6039/j.issn.1001-0408.2015.22.25
- VernacularTitle:芫花甘草反药组合对小鼠肝脏损伤的机制研究
- Author:
Yi WANG
;
Yuan AN
;
Haibin WU
- Publication Type:Journal Article
- Keywords:
Daphne genkwa;
Glycyrrhiza uralensis;
Liver damage;
Combination of incompatible drugs;
Transporter Ntcp;
Mice
- From:
China Pharmacy
2015;(22):3106-3108
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the liver damage mechanism in mice caused by the incompatibility of Daphne genkwa com-bined with Glycyrrhiza uralensis in aspect of liver transporter. METHODS:40 mice were equally randomized into a normal control (isometric normal saline)group,a group of G. uralensis [15 g(medicinal matierias)/kg],a group of D. genkwa [15 g(medicinal tatierias)/kg],a group of D. genkwa with G. uralensis in the ratio of 1∶1 [15 g(crude drug)/kg],a group of D. genkwa with G. uralensis in the ratio of 1∶3 [15 g(medicinal matierias)/kg](n=8). The mice were given the corresponding drug,ig,once a day for 7 consecutive days. HE staining was performed and then the pathomorphology of liver tissues were observed under the light mi-croscope,and calculation was made for pathological grading. Western blot method was employed to determine the protein expre-ssion of the transporter Ntcp protein in the livers of mice. The contents of total bile acids(TBA)in livers of mice were determined. RESULTS:Compared to the mice in the normal control group,those in the group of 1∶1 and 1∶3 demonstrated higher protein ex-pression of Ntcp. In the group of 1∶1,the mice with grade“+++”hepatocyte degeneration were more (8). The mice with grade“+++”and“++”hepatocyte degeneration in the groups of 1∶3 were more (2 and 8 respectively). CONCLUSIONS:D. genkwa combined with G. uralensis can induce liver damage in mice by a mechanism which may be related to the accumulation of a large amount of TBA in the liver as a result of the increase in the expression of Ntcp in mice.
