Study of Mechanism on in vitro Permeation of Phillyrin through Blood-brain Barrier
10.6039/j.issn.1001-0408.2015.22.08
- VernacularTitle:连翘苷经血脑屏障体外透过机制研究
- Author:
Haiying HUANG
;
Chengfu SU
;
Yanli GUO
;
Yongheng WEI
;
Beiyue ZHANG
;
Jinli SHI
- Publication Type:Journal Article
- Keywords:
MDR1 transfection;
Madin-Darby canine kidney epithelial cell;
Blood-brain barrier;
Phillyrin;
Cell tight junction protein;
in vitro test
- From:
China Pharmacy
2015;(22):3049-3051
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To research the mechanism of in vitro permeation of phillyrin through blood-brain barrier. METH-ODS:After Madin-Darby canine kidney epithelial cells transfected with colorectal cancer MDR1 gene (MDCK-MDR1) were cul-tured with phillyrin solution of 0(negative control),10,25,50,75 and 100μg/ml for 24 h,cell viability was determined by resa-zurin method and cell survival rate was calculated. After MDCK-MDR1 cells were cultured with phillyrin solution of 10,25,50, 75 and 100 μg/ml for 10 min,the content of phillyrin in the cells was determined,and concentration-uptake rate curve was drawn. Following 3 h culture of MDCK-MDR1 cells with phillyrin solution of 0 (negative control),50 and 100 μg/ml,the structure of cell tight junction protein was observed under the inverted microscope. RESULTS:Compared to the negative control group,after 24 h cell culture with phillyrin solution of 10-100 μg/ml,no obvious change in cell survival rate occurred. MDCK-MDR1 cells cultured with the phillyrin at a mass concentration of 10-100 μg/ml demonstrated a nonlinear relationship with concentration of phillyrin and a gradual saturation trend. After the cells were cultured with phillyrin of 50 and 100 μg/ml for 3 h,cell tight junction protein was intact. CONCLUSIONS:The absorption of phillyrin through the simulated blood-brain barrier may be in the form of passive transportation combined with active transportation,the concertration has effect on cell tight junction protein.
