Establishment of Mouse Models with Kidney-positive Deficiency with Antisense Oligonucleotide-loaded Bio-degradable Polylactic Acid Microspheres
10.6039/j.issn.1001-0408.2015.22.19
- VernacularTitle:采用反义寡核苷酸聚乳酸可降解微球建立小鼠肾阳虚模型
- Author:
Xiaoqing LI
;
Jian YING
- Publication Type:Journal Article
- Keywords:
Antisense oligonucleotide;
Biodegradable polylactic acid microspheres;
Mouse;
Kidney-positive deficiency;
Gluco-corticoid receptor
- From:
China Pharmacy
2015;(22):3086-3088
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the feasibility of mouse models establishment with kidney-positive deficiency with anti-sense oligonucleotide-loaded biodegradable polylactic acid microspheres. METHODS:Three-week old mice were randomized into groups A,B,C,D and E,with 6 mice in each group,where the mice in group A were given subcutaneous injection 0.2 ml nor-mal saline,and those in groups B-E were given subcutaneous injection 0.1,0.2,0.4 and 0.8 mg each mice of antisense oligonucle-otide-loaded biodegradable polylactic acid microspheres. After the administration,the weights and activities of mice in all groups were observed;and one month later,their livers,adrenal glands and brains were collected to detect the expression of glucocorti-coid receptor (GR) by real-time quantitative polymerase chain reaction (RT-PCR). RESULTS:All mice except those in group A were found to have kidney-positive deficiency symptoms such as developmental delay,light weight,less activities and gathering to-gether. Compared to group A,other groups showed lower GR expressions in liver,adrenal gland and brain. There was statistically difference (P<0.05). CONCLUSIONS:Antisense oligonucleotide-loaded biodegradable polylactic acid microspheres can inhibit GR expression,and the mice whose GR expression is inhibited show symptoms of kidney-positive deficiency,which provides a re-ference for the establishment of mouse models with kidney-positive deficiency.
