Inhibitory Effects of Ampelopsin Sodium Combined with Carboplatin on the Proliferation of Lewis Cell of Lung Cancer
10.6039/j.issn.1001-0408.2015.19.22
- VernacularTitle:蛇葡萄素钠协同卡铂对肺癌Lewis细胞增殖的抑制作用
- Author:
Miaomiao MIAO
;
Yanxia ZHANG
;
Yongjie WU
;
Hong QIN
- Publication Type:Journal Article
- Keywords:
Ampelopsin sodium;
Carboplatin;
Lewis cells of lung cancer;
Caspase-3
- From:
China Pharmacy
2015;(19):2664-2666
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the inhibitory effects of ampelopsin sodium (AMP-Na) combined with carboplatin on the proliferation of Lewis cell of lung cancer. METHODS:6.25,12.5,25,50 and 100 μg/ml AMP-Na and 3.125,6.25,12.5,25 and 50 μg/ml carboplatin were used to culture the cells for 4 h,and the cell viability was determined and the inhibition rate and half in-hibition concentration(IC50)were calculated. 12.5,25,50 and 100 μg/ml AMP-Na and 12.5 μg/ml carboplatin were used to culture the cells for 12 h,and the flow cytometry was used to determine the expression of Caspase-3. RESULTS:AMP-Na with serial con-centrations combined with carboplatin with serial concentrations had obvious inhibitory effects on the cell proliferation. With the in-crease of mass concentration,the IC50 of carboplatin on the Lewis cells was gradually decreased;when the AMP-Na of 6.25-50μg/ml was combined with carboplatin of 3.125-25 μg/ml,it showed the strongest inhibitory effects on the Lewis cell proliferation. When cells were cultured with AMP-Na and carboplatin for 24 h,the expression of Caspase-3 increased significantly. CONCLU-SIONS:AMP-Na combined with carboplatin has synergistic inhibitory effect on the Lewis cell proliferation by a mechanism that may be related to the apoptosis induced by Caspase-3 activated by AMP-Na.
