Study on Extraction Technology of Polysaccharides from Stemona tuberosa by Alkaline Water Ultrasound Method
10.6039/j.issn.1001-0408.2015.19.32
- VernacularTitle:碱水超声波法提取对叶百部粗多糖的工艺研究
- Author:
Chang WANG
;
Liyan CHEN
;
Xiaoxue SUN
;
Shuming ZHANG
;
Wenfeng WEI
;
Weiming WANG
- Publication Type:Journal Article
- Keywords:
Stemona tuberosa;
Polysaccharide;
Alkaline water ultrasound method;
Extraction technology
- From:
China Pharmacy
2015;(19):2695-2697
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimize extraction technology of polysaccharides from Stemona tuberosa so as to increase the ex-traction rate and efficiency. METHODS:Anthrone-sulfuric acid method was used for detection. With water and alkaline water (NaOH solution)as the solvent respectively,the effects of decocting method,ultrasound method and microwave method on the ex-traction rate of crude polysaccharide were observed respectively. By carrying out single factor and orthogonal tests,with the extrac-tion rate of crude polysaccharide as the index,the effects of the amount of water,NaOH solution concentration,extraction tempera-ture and extraction time on the extraction of crude polysaccharide by alkaline water ultrasound method were observed to optimize the technology parameters,and verification test was conducted. RESULTS:The alkaline water ultrasound method is superior to oth-er extraction methods,where the optimal technology parameters were as follows as 0.3 mol/L NaOH aqueous solution 30 times as much as the amount of crud drug,ultrasonic processing for 70 min at 50 ℃. The verification tests showed that the average extrac-tion rate of crude polysaccharide was 25.76%(RSD=3.51%,n=6). CONCLUSIONS:The optimal alkaline water ultrasound method can better extract crude polysaccharide from S. tuberosa.
