Optimization of Extraction Technology of Polysaccharides from Cipangopaludina chinesis by Enzyme Method
10.6039/j.issn.1001-0408.2015.19.36
- VernacularTitle:酶法提取中国圆田螺多糖的工艺优选
- Author:
Xiaoyan LIU
;
Chaopin LI
;
Tao ZHU
- Publication Type:Journal Article
- Keywords:
Cipangopaludina chinesis;
Polysaccharide;
Papain;
Extraction technology;
Orthogonal test
- From:
China Pharmacy
2015;(19):2708-2710
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimize the extraction technology of polysaccharides from Cipangopaludina chinesis by enzyme method. METHODS:The papain enzymolysis extraction was performed. On the basis of single factors tests,taking the yield of polysaccharides as the index,four factors including enzyme concentration,pH,enzymolysis temperature and enzymolysis time in the extraction process were optimized by the orthogonal test,and verification was carried out. RESULTS:The optimal technology by enzyme method was as follows as enzyme concentration of 1.0%,pH of 7.0,enzymolysis temperature of 55 ℃ and enzymoly-sis time of 3.0 h. Under this technology,the average yield of polysaccharides was 4.98%(RSD=2.51%,n=3)and the content of polysaccharides was 73.54%(RSD=1.36%,n=3). CONCLUSIONS:The optimal extraction technology of polysaccharides from C. chinesis was simple,reasonable and feasible.
