Regulation Effects of Oleanolic Acid on the Mitochondrial Nitric Oxide Synthase in Human Umbilical Vein Endothelial Cells with Oxidative Damage
10.6039/j.issn.1001-0408.2015.19.08
- VernacularTitle:齐墩果酸对氧化损伤人脐静脉内皮细胞线粒体中一氧化氮合酶的调控作用Δ
- Author:
Yu WANG
;
Zhiwu HAN
- Publication Type:Journal Article
- Keywords:
Oleanolic acid;
Endothelial cells;
Mitochondria;
Nitric oxide synthase;
Reactive oxygen species
- From:
China Pharmacy
2015;(19):2617-2620
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the regulation effects of oleanolic acid on the mitochondrial nitric oxide synthase(mt-NOS) in human umbilical vein endothelial cells (HUVECs) with oxidative damage. METHODS:HUVECs in exponential phase were divided into normal group,model group and oleanolic acid low,medium and high dose groups(5,20 and 35 μmol/L). After drug acting for 24 h,all groups were given culture solution containing 100 μg/ml ox-LDL to reproduce oxidative damage except normal group. CCK-8 was used to detect cell viability. The mitochondria in cells were extrated,enzyme chemical method was used to detect mtNOS activity and mtNO content,fluorescence microplate method was used to detect fluorescence intensity of reactive oxygen species(ROS),and western blot was used to detect expression of cytochrome C(Cyto-C). RESULTS:Compared with nor-mal group,the cell viability in model group was decreased;mtNOS activity,mtNO content,ROS fluorescence intensity and Cy-to-C protein expression were increased,with significant differences (P<0.05). Compared with model group,the cell viability in oleanolic acid low,medium and high dose groups was increased;mtNOS activity,mtNO content,ROS fluorescence intensity and Cyto-C protein expression were decreased,with significant differences(P<0.05),and they had positive correlation with concentra-tions. CONCLUSIONS:Oleanolic acid can decrease the mtNOS activity of HUVECs,reduce the production of mtNO and Cyto-C, by a mechanism that may be related to the decrease of ROS expression.
