Differential Protein Expressions in Virus-Infected and Uninfected Trichomonas vaginalis.
10.3347/kjp.2017.55.2.121
- Author:
Ding HE
1
;
Gong PENGTAO
;
Yang JU
;
Li JIANHUA
;
Li HE
;
Zhang GUOCAI
;
Zhang XICHEN
Author Information
1. College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China. Jianhuali7207@163.com, xczhang@jlu.edu.cn
- Publication Type:Original Article
- Keywords:
Trichomonas vaginalis;
Trichomonas vaginalis virus;
iTRAQ;
quantitative PCR;
protein expression
- MeSH:
Glucose-6-Phosphate Isomerase;
Glycogen Phosphorylase;
Heat-Shock Proteins;
Host-Parasite Interactions;
Malate Dehydrogenase;
Metabolism;
Polymerase Chain Reaction;
Proteome;
Reticuloendotheliosis virus;
Ribosomal Proteins;
RNA, Double-Stranded;
RNA, Messenger;
Trichomonas vaginalis*;
Trichomonas*;
Triose-Phosphate Isomerase;
Virulence
- From:The Korean Journal of Parasitology
2017;55(2):121-128
- CountryRepublic of Korea
- Language:English
-
Abstract:
Protozoan viruses may influence the function and pathogenicity of the protozoa. Trichomonas vaginalis is a parasitic protozoan that could contain a double stranded RNA (dsRNA) virus, T. vaginalis virus (TVV). However, there are few reports on the properties of the virus. To further determine variations in protein expression of T. vaginalis, we detected 2 strains of T. vaginalis; the virus-infected (V⁺) and uninfected (V⁻) isolates to examine differentially expressed proteins upon TVV infection. Using a stable isotope N-terminal labeling strategy (iTRAQ) on soluble fractions to analyze proteomes, we identified 293 proteins, of which 50 were altered in V⁺ compared with V⁻ isolates. The results showed that the expression of 29 proteins was increased, and 21 proteins decreased in V⁺ isolates. These differentially expressed proteins can be classified into 4 categories: ribosomal proteins, metabolic enzymes, heat shock proteins, and putative uncharacterized proteins. Quantitative PCR was used to detect 4 metabolic processes proteins: glycogen phosphorylase, malate dehydrogenase, triosephosphate isomerase, and glucose-6-phosphate isomerase, which were differentially expressed in V⁺ and V⁻ isolates. Our findings suggest that mRNA levels of these genes were consistent with protein expression levels. This study was the first which analyzed protein expression variations upon TVV infection. These observations will provide a basis for future studies concerning the possible roles of these proteins in host-parasite interactions.
