Content Determination of Paeoniflorin and Liquoritin in Jiawei Xiaoyao Pills by HPLC
10.6039/j.issn.1001-0408.2015.18.44
- VernacularTitle:HPLC法测定加味逍遥丸中芍药苷与甘草苷的含量
- Author:
Rong DU
;
Mengyou ZHANG
- Publication Type:Journal Article
- Keywords:
HPLC;
Jiawei xiaoyao pills;
Paeoniflorin;
Liquoritin
- From:
China Pharmacy
2015;(18):2571-2572
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the HPLC method for the content determination of paeoniflorin and liquoritin in Jiawei xiaoyao pills. METHODS:It was performed on Hypersil-ODS C18 with the mobile phase of acetonitrile-water(14∶86,V/V)at the flow rate of 1.0 ml/min,the detection wavelength was 230 nm,the column temperature was room temperature and the volume was 10 μl. RESULTS:The linear range was 16.12-80.6 μg/ml for paeoniflorin(r=0.999 6)and 6.08-30.4 μg/ml for liquoritin(r=0.999 8);the RSDs of precision,repeatability and stability tests were all less than 2.0%;the average recovery was respectively 98.51%(RSD=1.94%,n=6)and 98.08%(RSD=1.29%,n=6). CONCLUSIONS:The method is simple,accurate and reliable, and can be used for the content determination of paeoniflorin and liquoritin in Jiawei xiaoyao pills.
