Application of Fluorescence Quantitative RT-PCR for Live-attenuated Mumps Vaccine Manufacturing

VernacularTitle:荧光定量RT-PCR滴度检测法在腮腺炎减毒活疫苗制造中的应用
Author: Jingwu ZHANG ; Xianchao LIU
Publication Type:Journal Article
Keywords: Live-attenuated mumps vaccine; S79 strain; Fluorescence quantitative reverse transcription-polymerase chain reac-tion; Titer of virus
From: China Pharmacy 2015;(18):2578-2580
CountryChina
Language:Chinese
Abstract: OBJECTIVE:To provide reference for improving the practical application of fluorescence quantitative reverse tran-scription-polymerase chain reaction (RT-PCR) for the live-attenuated mumps vaccine manufacturing. METHODS:A regression equation of standard titer-ct value was established;micro cytopathic effect(micro-CPE)and fluorescence quantitative RT-PCR were respectively adopted for the titer of 10 batches of single harvest liquid of live-attenuated mumps vaccine and end products,and the results were compared. RESULTS:According to the t-test analysis,the P were respectively 0.743 and 0.868,and the average val-ues were in the range of (-0.174,0.094) and (-0.113,0.153),with no significant differences. They were similar. CONCLU-SIONS:Fluorescence quantitative RT-PCR can be used for the titer detection of single harvest liquid and end products in the pro-cess of live-attenuated mumps vaccine manufacturing. And it is expected to further upgrade to Chinese Pharmacopoeia method.