Content Determination of Eudesmin in Zanthoxylum simulans by HPLC
10.6039/j.issn.1001-0408.2015.33.28
- VernacularTitle:HPLC法测定麻口皮子药中桉脂素的含量
- Author:
Shuai PENG
;
Weijun HE
;
Nianzhen LIU
;
Xiaojiang ZHOU
- Publication Type:Journal Article
- Keywords:
Zanthoxylum simulans;
HPLC;
Content determination;
Eudesmin
- From:
China Pharmacy
2015;26(33):4688-4689
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of eudesmin in Zanthoxylum simulans. METH-ODS:HPLC was performed on the column of Kromasil C18 with mobile phase of acetonitrile-water(56:44,V/V)at flow rate of 1.0 ml/L,detection wavelength was 221 nm,column temperature was 25 ℃. RESULTS:The linear of eudesmin was 0.208-2.08 μg (r=0.999 8);RSDs of precision,reproducibility and stability tests were lower than 2.0%;recovery was 96.18%-103.04%(RSD=2.58,n=6). CONCLUSIONS:The method is simple and good reproducibility,and can be used for the content determination of eu-desmin in Z. simulans.
