Study on the HPLC Fingerprint Spectrum of Mongolian Medicine Rhaponticum uniflorum
10.6039/j.issn.1001-0408.2015.33.29
- VernacularTitle:蒙药漏芦花的HPLC指纹图谱研究
- Author:
Xiang TIAN
;
Meili WANG
;
Yuxia BAI
- Publication Type:Journal Article
- Keywords:
Mongolian medicine;
Rhaponticum uniflorum;
HPLC;
Fingerprint spectrum
- From:
China Pharmacy
2015;26(33):4690-4692
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish HPLC fingerprint sectrum of Rhaponticum uniflorum. METHODS:HPLC was performed on the column of Hypersil-ODS with mobile phase of 0.3% phosphoric acid-acetonitrile(gradient elution) at flow rate of 1.0 ml/min,detection wavelength was 220 nm,column temperature was 30 ℃ and volume injection was 10 μl. The luteolin was refer-ence,17 batches of R. uniflorum from different production places was analyzed and similarity evaluation system for chromatograph-ic fingerprint of TCM (2004 A edition)was adopted for the similarity analysis. RESULTS:There were totally 11 common peaks with similarity degree≥0.900 of 17 batches. According to the verification,the fingerprint spectrum and reference fingerprint spec-trum of R. uniflorum had good consistency. CONCLUSIONS:The established method is specific and stable,and can provide refer-ence for the identification and quality control of R. uniflorum.
