Study on the Quality Standards for Gastrodia elata Horey-fired Tablet
10.6039/j.issn.1001-0408.2015.33.33
- VernacularTitle:天麻蜜制片的质量标准研究
- Author:
Aiping ZUO
;
Chuanfang WANG
;
Mei PAN
;
Jingwen TANG
;
Liyan ZHANG
;
Hao WU
;
Jianping GONG
- Publication Type:Journal Article
- Keywords:
Gastrodia elata hency-fired tablet;
Quality standard;
HPLC;
Gastrodin;
Benzyl alcohol
- From:
China Pharmacy
2015;26(33):4703-4705
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the quality standard for Gastrodia elata hency-fired tablet. METHODS:TLC was used to identify the gastrodin and benzyl alcohol and determine the content of moisture,ash and extract;HPLC was used to determine the content of gastrodin and benzyl alcohol. Column was Diamonsil C18 with mobile phase of acetonitrile-0.05% phosphate(3:97,V/V) at flow rate of 1.0 ml/min,detection wavelength was 220 nm,column temperature was 25 ℃ and volume injection was 10 μl. RE-SULTS:The TLC of gastrodin and benzyl alcohol showed clear spots and good separation. The moisture content<35.0%,total ash contents<2.0% and extract content>40.0%. Linear range of gastrodin was 25.2-126.0,12.7-63.5 μg/ml(r=0.999 9),respectively;RSDs of precision,reproducibility and stability tests were lower than 2.0%;recovery was 99.49%-102.40%(RSD=1.09%,n=6), 98.75-102.63%(RSD=1.53,n=6),respectively. CONCLUSIONS:The method is simple and good reproducibility,and can be used for the quality control of Gastrodia elata hency-fired tablet.
