Comparative Study on the Main Medical Component Contents of Angelica pubescens in Famous Region Chongqing and Other Domestic Areas in China
10.6039/j.issn.1001-0408.2015.33.36
- VernacularTitle:重庆道地药材独活与全国其他产区独活主要药效成分含量对比研究
- Author:
Taide HU
;
Shaocheng CHEN
;
Jun TAN
- Publication Type:Journal Article
- Keywords:
Famous region drug;
Angelica pubescens;
Osthole;
Columbianadin;
HPLC
- From:
China Pharmacy
2015;26(33):4711-4713
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To compare the contents of osthole and columbianadin in Argelica. pubescens in Wushan county, Wuxi county in Chongqing and other domestic areas in china. METHODS:HPLC was performed on the column of Shiseido Cap-cell Pak C18 with mobile phase of acetonitrile-water(gradient elution) at flow rate of 1.0 ml/min,detection wavelength was 330 nm,column temperature was 30 ℃ and volume injection was 10 μl. RESULTS:The linear range was 0.015 1-0.301 6 mg/ml(r=0.999 9) for osthole and 0.016 7-0.334 4 mg/ml(r=0.999 9) for columbianadin;RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoveries were 98.46%-106.11%(RSD=2.34%,n=9)and 98.24%-102.69%(RSD=1.29%,n=9), respectively. CONCLUSIONS:The method is simple and good reproducibility,and and can be used for the contents determination of osthole and columbianadin in A. pubescens. The contents of osthole and columbianadin in A. pubescens of Wushan county in Chongqing are obviously higher than Wuxi county and other domestic areas in China.
