Simultaneous Determination of Paeoniflorin and Ferulic Acid in Naoxueshuan Pill by HPLC
10.6039/j.issn.1001-0408.2015.33.37
- VernacularTitle:HPLC法同时测定脑血栓丸中芍药苷和阿魏酸的含量
- Author:
Jianping LIU
;
Tao CHEN
- Publication Type:Journal Article
- Keywords:
Naoxueshuan pill;
Paeoniflorin;
Ferulic acid;
HPLC
- From:
China Pharmacy
2015;26(33):4714-4716
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the simultaneous determination of paeoniflorin and ferulic acid in Naoxuesh-uan pill. METHODS:HPLC was performed on the column of Waters SunfireTM C18 with mobile phase of acetonitrile-water-glacial acetic acid(17:82:1,V/V/V)at a flow rate of 1.0 ml/min,detection wavelength was 230 nm,column temperature was 35 ℃,and the volume injection was 20 μl. RESULTS:The linear range was 0.024-0.240 mg/ml for paeoniflorin(r=0.999 9)and 0.009 4-0.141 0 mg/ml(r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 95.00-97.90%(RSD=0.59%,n=6) and 95.29-98.24%(RSD=0.54%,n=6),respectively. CONCLUSIONS:The method is simple and repro-ducible,and can be used for the quality control of Naoxueshuan pill.
