Study on the GC-MS Fingerprint Spectrum of Volatile Components in Danqi Piantan Capsule
10.6039/j.issn.1001-0408.2015.33.38
- VernacularTitle:丹芪偏瘫胶囊挥发性成分的GC-MS指纹图谱研究
- Author:
Jiejin SONG
;
Tao CHEN
;
Jin LI
- Publication Type:Journal Article
- Keywords:
Danqi piantan capsule;
Volatile components;
Fingerprint;
GC-MS;
Quality control
- From:
China Pharmacy
2015;26(33):4716-4718
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the GC-MS fingerprint spectrum of volatile components in Danqi piantan capsule. METH-ODS:GC-MS was performed. HPLC conditions:The column was BPX 5,temperature of injection was 250 ℃,respectively;carri-er gas was He,flow rate was 1 ml/min,split injection,split ratio was 50:1 and volume injection was 0.02 μl. MS conditions:ion source was EI,bombarding energy was 70 eV,temperature of ion source and transmission line was 200 ℃ and 250 ℃,respective-ly;scan range was 40-500 amu,solvent delay of 2 min. The peak with largest chromatographic peaks areas was reference peak,com-mon peaks of 10 batches of Danqi piantan capsule were analyzed. Similarity evaluation system for chromatographic figerprint of TCM was adapted for the similarity analysis and NIST standard MS database was used to retrieve and identify the common peak. RESULTS:There were totally 13 common peaks in the 10 batches of Danqi piantan capsule with similarity degree>0.92;accord-ing to the verification,the fingerprint spectrum was a dapted for the similarity analysis and reference fingerprint of 10 batches of Danqi piantan capsule showed good consistency. The No.3 peak was Angelica sinensis unique,the No.2,4,5,7,8,12 peak was Acorus tatarinowii unique. CONCLUSIONS:The established method is specific and stable,and can provide basis for the quality as-sessment for Danqi piantan capsule.
