Simultaneous Contents Determination of Tetrahydropalmatine and Rosmarinic Acid in Xiaoheling Capsule by HPLC
10.6039/j.issn.1001-0408.2015.33.39
- VernacularTitle:HPLC法同时测定消核灵胶囊中延胡索乙素和迷迭香酸的含量
- Author:
Dianrong WANG
;
Hongxi CHEN
;
Zhihui YANG
- Publication Type:Journal Article
- Keywords:
Xiaoheling capsule;
Tetrahydropalmatine;
Rosmarinic acid;
HPLC
- From:
China Pharmacy
2015;26(33):4719-4721
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the contents determination of tetrahydropalmatine and rosmarinic acid in Xiaoheling capsule. METHODS:HPLC was performed on the column of Phenomenex C18 with mobile phase A of 0.1% phosphoric acid(adjusted pH to 6.0 with triethylamine)-methanol(45:55,V/V) and (adjusted pH to 6.0 with triethylamine) B of 0.1% phos-phoric acid-methanol(55:45,V/V)(gradient elution)at flow rate of 0.8 ml/min,detection wavelength was 282 nm(0-25 min)and 330 nm(25.01-60 min)and volume size was 10 μl. RESULTS:The linear range was 1.502-75.10 μg/ml for tetrahydropalmatine and 6.03-301.6 μg/ml for rosmarinic acid(r=0.999 8);RSDs of precision,reproducibility and stability tests were ≤1.36%;recoveries were 97.11-99.87%(RSD=0.95%,n=9)and 98.47-101.99%(RSD=1.08%,n=9),respectively. CONCLUSIONS:The method is simple,accurate and reliable,and can be used for contents determination of tetrahydropalmatine and rosmarinic acid in Xiaoheling capsule.
