Simultaneous Determination of Hydroxysafflor Yellow A and Aalvianolic Acid B in Compound Danshen Oral Solution by HPLC
10.6039/j.issn.1001-0408.2015.33.42
- VernacularTitle:HPLC法同时测定复方丹参口服液中羟基红花黄色素A和丹酚酸B的含量
- Author:
Guanliang PENG
;
Zhao MEI
;
Lingti KONG
- Publication Type:Journal Article
- Keywords:
Compound danshen oral solution;
Hydroxysafflor yellow A;
Salvianolic acid B;
HPLC
- From:
China Pharmacy
2015;26(33):4726-4728
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for simultaneous determination the contents determination of hydroxysafflor yel-low A and salvianolic acid B in Compound danshen oral solution. METHODS:HPLC was performed on the column of SHIMADZU ODS-C18 with mobile phase of methanol-0.5% phosphoric acid (35:65,V/V) at flow rate of 1.0 ml/min,dection wavelength was 403 nm for hydroxysafflor yellow A and 286 nm for salvianolic acid B and column temperature was 35 ℃, the volume injection was 20μl. RESULTS:The linear range was 2.01-20.10μg/ml for hydroxysafflor yellow A(r=0.999 9)and 39.80-398.00μg/ml(r=0.999 9) for salvianolic acid B. RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 98.26%-101.25%(RSD=0.94%,n=9)and 98.70%-101.35%(RSD=0.71%,n=9),respectively. CONCLUSIONS:The method is feasible and reproducible,and can be used for the contents determination of hydroxysafflor yellow A and salvianolic acid B in Com-pound danshen oral solution.
