Simultaneous Determination of the Content of 4 Psoralen Compounds in Buwu Tincture by HPLC
10.6039/j.issn.1001-0408.2015.33.45
- VernacularTitle:HPLC法同时测定补乌酊中4种补骨脂类成分的含量
- Author:
Tianyi XIA
;
Shun CHEN
;
Shouhong GAO
;
Chunhua YOU
;
Feng ZHANG
;
Wenquan LU
;
Shujuan PIAO
;
Wansheng CHEN
- Publication Type:Journal Article
- Keywords:
HPLC;
Buwu tincture;
Angelicin;
Bavachin;
Psoralidin;
Isobavachalcone;
Content
- From:
China Pharmacy
2015;26(33):4734-4736
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for simultaneous determination of 4 psoralen compounds in Buwu tincture. METHODS:HPLC was performed on the column of Dikma Diamonsil C18 with mobile phase of acetonitrile-0.2% Acetic acid by gradient elution at flow rate of 1 ml/min,detection wavelength was 246 nm,column temperature was 30 ℃,and the injection vol-ume was 15 μl. RESULTS:The linear range was 13.00-208 μg/ml for angelicin,26.00-416 μg/ml for bavachin,24.50-392 μg/ml for psoralidin and 37.88-606 μg/ml for isobavachalcone,respectively(r≥0.999 6);RSDs of precision,stability and reproducibility tests were less than 2.00%;recoveries were 95.22%-97.23%(RSD=0.87%,n=6),100.24%-104.64%(RSD=1.62%,n=6), 102.28%-104.39%(RSD=1.47%,n=6)and 97.68%-100.17%(RSD=0.97%,n=6),respectively. CONCLUSIONS:The method is simple and reproducible,and can be used for the quality control of Buwu tincture.
