Simultaneous Determination of 5 Components in Qingwei Huanglian Pill by HPLC
10.6039/j.issn.1001-0408.2015.33.47
- VernacularTitle:HPLC法同时测定清胃黄连丸中5种成分的含量
- Author:
Aili MAO
- Publication Type:Journal Article
- Keywords:
HPLC;
Qingwei huanglian pill;
Geniposide;
Paeoniflorin;
Berberine hydrochloride;
Baicalin;
Paeonol;
Content determination
- From:
China Pharmacy
2015;26(33):4740-4742
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the simultaneous determination of geniposide,paeoniflorin,berberine hy-drochloride,baicalin and paeonol in Qingwei huanglian pill. METHODS:HPLC was performed on the column of Diamonsil C18 with mobile phase of acetonitrile-0.3% phosphate(gradient elution)at flow rate of 1.0 ml/min,detection wavelength was 238 nm, column temperature was 30 ℃ and the injection volume was 10 μl. RESULTS:The linear range was 15.36-153.6 μg/ml for genipo-side, 6.56-65.6 μg/ml for paeoniflorin, 22.74-227.4 μg/ml for berberine hydrochloride, 26.06-260.6 μg/ml for baicalin and 5.57-55.7 μg/ml for paeonol(r≥0.999 8);RSDs of precision,stability and reproducibility tests were not higher than 1.0%;recov-eries were 96.28%-99.38%(RSD=1.1%,n=6),97.13%-99.48%(RSD=1.2%,n=6),98.14%-100.25(RSD=0.7%,n=6), 97.38%-100.05%(RSD=1.0%,n=6) and 96.30%-99.12%(RSD=1.2%,n=6),respectively. CONCLUSIONS:The method is simple and good reproducibility,and suitable for the contents determination of geniposide,paeoniflorin,berberine hydrochloride, baicalin and paeonol in Qingwei huanglian pill.
