Content Determination of 4 Ingredients in Ligustrum lucidum Decoration Piece by Reference Extract Method
10.6039/j.issn.1001-0408.2015.33.27
- VernacularTitle:对照提取物法测定女贞子饮片中4种成分的含量
- Author:
Yi TAO
;
Chang ZHANG
;
Weidong LI
;
Tulin LU
;
Baochang CAI
- Publication Type:Journal Article
- Keywords:
Ligustrum lucidum;
Reference extract method;
HPLC
- From:
China Pharmacy
2015;26(33):4685-4687
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the contents determination of 4 ingredients in Ligustrum lucidum decoration piece by reference extract method. METHODS:HPLC was performed on the column of XBridge C18 with mobile phase of acetoni-trile-0.1% formic acid(gradient elution) at flow rate of 1.0 ml/min,detection wavelength was 254 nm,column temperature was 30℃and volume injection was 10μl. RESULTS:The linear range was 0.037-0.598 mg for neonuezhenide,0.006-0.101 mg for ac-teoside,0.189-3.023 mg for nuezhenide and 0.314-5.027 mg for specnuezhenide(r≥0.999 0);RSDs of precision,reproducibility and stability tests were no more than 3.8%;recoveries were 98.46%-104.83%(RSD=2.43,n=6),95.55%-104.57%(RSD=3.63, n=6),100.09%-104.39%(RSD=1.45,n=6)and 98.84%-104.97%(RSD=2.02,n=6). CONCLUSIONS:The method is simple, good reproducibility,and can be used for the contents determination of 4 ingredients in L. lucidum decoration piece.
