Content Determination of Geniposide in TCM Callus Convenient Wipes Drug Solution by HPLC
10.6039/j.issn.1001-0408.2015.33.32
- VernacularTitle:HPLC法测定中药愈伤便捷湿巾药液中栀子苷的含量
- Author:
Lulu YANG
;
Xueling QIN
;
Jing PENG
;
Yagong NING
- Publication Type:Journal Article
- Keywords:
HPLC;
TCM callus convenient wipes;
Drug slution;
Geniposide;
Content determination
- From:
China Pharmacy
2015;26(33):4701-4703
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of geniposide in TCM callus convenient wipes drug solution. METHODS:HPLC was performed on the column of Agilent Zorbax SB-C18 with mobile phase of acetonitrile-0.1%phosphoric(10:90,V/V)at the flow rate of 1.0 ml/min,column temperature was 30 ℃,detection wavelength was 236 nm and the volume was 10 μl. RESULTS:The linear range of geniposide was 0.104 1-1.041 μg (r=0.999 8);RSDs of precision,stability and reproducibility tests were all lower than 2%;recovery was 99.04%-100.82%(RSD=0.85%,n=6). CONCLUSIONS:The method is simple,accurate,reproducible,and can be used for the content determination of geniposide in TCM callus convenient wipes drug solution.
