Content Determination of Diosgenin in Paris polyphylla var. yunnanensis from 14 Different Habitats by HPLC
10.6039/j.issn.1001-0408.2015.21.31
- VernacularTitle:HPLC法测定14个不同产地滇重楼中薯蓣皂苷元的含量Δ
- Author:
Jing LUO
;
Yuxiang SHEN
;
Nong ZHOU
;
Liping DENG
- Publication Type:Journal Article
- Keywords:
Paris polyphylla var. yunnanensis;
Diosgenin;
HPLC;
Content determination
- From:
China Pharmacy
2015;(21):2965-2967
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of diosgenin in Paris polyphylla var. yunnanensis and compare the contents in Paris polyphylla var. yunnanensis among different habitats. METHODS:HPLC was performed on the column of Intersil ODS-C18 with mobile phase of acetonitrile-water(92∶8,V/V)at the flow rate of 1.0 ml/min,the detection wave-length was 203 nm,column temperature was 35 ℃,and volume was 20 μl. RESULTS:The linear range of diosgenin was 0.539-13.475 μg(r=0.999 9);RSDs of precision,stability and reproducibility tests were all no more than 1.92%;average recov-ery was 98.48%(RSD=1.21%,n=6). The contents of diosgenin in P. polyphylla var. yunnanensis from 14 different habitats were in the range of 3.869 4-21.074 7 mg/g. CONCLUSIONS:The method is fast,simple,accurate and reliable,and can be used to de-termine the content of diosgenin in P. polyphylla var. yunnanensis. The diosgenin content can be greatly affected by habitat and envi-ronmental factors. P. polyphylla var. yunnanensis shows certain region-and habitat-dependent.
