Study on Standard Revision of Portulaca grandiflora
10.6039/j.issn.1001-0408.2015.21.36
- VernacularTitle:半枝莲的质量标准研究Δ
- Author:
Fuqiang LI
;
Kunjun MAO
;
Ping ZHOU
;
Dongdong CHEN
;
Xiang LI
;
Jianwei CHEN
- Publication Type:Journal Article
- Keywords:
Portulaca grandiflora;
Quality standard;
Scutellarin;
Letuolin;
TLC;
HPLC
- From:
China Pharmacy
2015;(21):2980-2982
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To improve the quality standard of Portulaca grandiflora. METHODS:TLC was used to identify the P. grandiflora with the reference of letuolin and apigenin. HPLC was performed on the column of Hanbon-C18 with the mobile phase of methanol-acid gradient[water-acetic acid(61∶4)] at the flow rate of 1.0 ml/min,the detection wavelength was 335 nm,the temperature was 35 ℃ and the volume was 10 μl. RESULTS:There was a good linear relationship in the quality concentration of scutellarin and luteolin respectively in th range of 0.299 5-1.872 0 μg(r=0.999 8)and 0.015 1-0.094 4 μg(r=0.999 5);the RSDs of precision,stability and reproducibility tests were ≤2.61%. The average recovery was respectively 100.42%(RSD=0.81%,n=6) and 100.65%(RSD=1.64%,n=6). CONCLUSIONS:The established TLC and HPLC are stable,reliable,simple and can be used for the qualitative and quantitative analysis of P. grandiflora.
