Exploration of the effect of Helq gene on stem cell pluripotency

VernacularTitle:Helq 对干细胞多能性影响的研究
Author: Cong WAN ; Yaping HUANG ; Mei WANG ; Yamei XIAO ; Xiaoyang ZHAO
Publication Type:Journal Article
Keywords: Helq; Pluripotency; Embryonic stem cells; Epiblast-like cells; CRISPR-Cas9
From: Acta Laboratorium Animalis Scientia Sinica 2016;24(4):358-363
CountryChina
Language:Chinese
Abstract: Objective To explore whether Helq deletion affect the pluripotency of stem cells. Methods Helq knockout embryonic stem cells were obtained by CRISPR?Cas9 gene editing technique. Results The results of immunoflu?orescence analysis showed that the expression of Oct4 and Nanog had no obvious difference to that of the control cells. The Helq-/ - embryonic stem cells could produce viable pups by tetraploid complementation, indicating that their pluripotency was not affected. Meanwhile, we found that day 2 epiblast?like cells also were obtained through differentiation of the Helq-/ - embryonic stem cells in vitro. Immunostaining and real?time PCR analysis showed that the gene expression of Helq-/ - epiblast cells were similar to the wild type cells. Conclusions Taken together, it is proved that the genomic in?stability caused by Helq deletion does not affect the pluripotency of pluripotent stem cells.