Isolation and identification of microbes from biofilm of Urinary catheters and antimicrobial Susceptibility evaluation
- Author:
Ajaranjit SINGH
- Publication Type:Journal Article
- Keywords:
Proteus mirabilis;
Biofilm;
antimicrobial activity;
urinary sample;
heart valve
- From:Asian Pacific Journal of Tropical Biomedicine
2012;(z3):1780-1783
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Bacterial species colonize indwelling catheters as biofilm induce complications in patients care. Methods: From the biofilm matrix seven species of microbes were isolated. The predominant bacteria seen in catheters were E.coli, (27 percent) P.mirabilis (20 percent) and S.epidermis (18 percent). Results: The biomass of microbes associated with the biofilm was estimated. The mean dry weight of biomass of bacteria associated with a catheter that was used for over a month time was in the range 2.5±0.04g - 3.1 ± 0.6g. Conclusion: But it was found to colonize the microtitre plate to attain a peak growth at 84h. P.mirabilis isolated from the biofilm was able to tolerate the antibiotics tetracycline, Penicillin, Kanamycin and Gentamycin at a dose level of 20μg/ml. The study indicated that the catheter has to be replaced if biofilm formation was noticed.
