Anti-oxidative and anti-inflammatory effects of Tagetes minuta essential oil in activated macrophages
10.1016/S2221-1691(14)60235-5
- Author:
Parastoo KARIMIAN
;
Gholamreza KAVOOSI
;
Zahra AMIRGHOFRAN
- Publication Type:Journal Article
- Keywords:
Tagetes minuta;
Essential oil;
Macrophages;
Anti-inflammatory;
Antioxidant
- From:Asian Pacific Journal of Tropical Biomedicine
2014;(3):219-227
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate antioxidant and anti-inflammatory effects of Tagetes minuta (T. minuta) essential oil.
Methods:In the present study T. minuta essential oil was obtained from leaves of T. minuta via hydro-distillation and then was analyzed by gas chromatography-mass spectrometry. The anti-oxidant capacity of T. minuta essential oil was examined by measuring reactive oxygen, reactive nitrogen species and hydrogen peroxide scavenging. The anti-inflammatory activity of T. minuta essential oil was determined through measuring NADH oxidase, inducible nitric oxide synthase and TNF-αmRNA expression in lipopolysacharide-stimulated murine macrophages using real-time PCR.
Results:Gas chromatography-mass spectrometry analysis indicated that the main components in the T. minuta essential oil were dihydrotagetone (33.86%), E-ocimene (19.92%), tagetone (16.15%), cis-β-ocimene (7.94%), Z-ocimene (5.27%), limonene (3.1%) and epoxyocimene (2.03%). The T. minuta essential oil had the ability to scavenge all reactive oxygen/reactive nitrogen species radicals with IC50 12-15 μg/mL, which indicated a potent radical scavenging activity. In addition, T. minuta essential oil significantly reduced NADH oxidase, inducible nitric oxide synthaseand TNF-αmRNA expression in the cells at concentrations of 50 μg/mL, indicating a capacity of this product to potentially modulate/diminish immune responses.
Conclusions:T. minuta essential oil has radical scavenging and anti-inflammatory activities and could potentially be used as a safe effective source of natural anti-oxidants in therapy against oxidative damage and stress associated with some inflammatory conditions.
